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The second response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - circular dichroism. As drawn in Fig - https://linktr.ee/olisclarity1. 9, the response may also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this could be due to the fact that the H4B radical serve as an electron sink in a later point in the reaction, so regarding allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo my sources intermediate that responds with NOHA.